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fibroblast growth factor 8/recombinant human/murine fgf-8b  (PeproTech)


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    PeproTech fibroblast growth factor 8/recombinant human/murine fgf-8b
    Fibroblast Growth Factor 8/Recombinant Human/Murine Fgf 8b, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+murine+fibroblast+growth+factor+(fgf)-2/8+ng+ml+bfgf/pm39772392-225-119-122
    Average 90 stars, based on 1 article reviews
    fibroblast growth factor 8/recombinant human/murine fgf-8b - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Antagonistic effects of IL-17 and Astragaloside IV on cortical neurogenesis and cognitive behavior after stroke in adult mice through Akt/GSK-3β pathway
    Article Snippet: NSCs were cultured in Neurobasal medium supplemented with 2% B27, 1% N2, 2% Gln, 20 ng/ml recombinant murine epidermal growth factor (EGF, Peprotech, Cat# 315-09), and 20 ng/ml recombinant murine fibroblast growth-basic factor (FGF, Peprotech, Cat# 450-33) for 7–10 days .

    Article Title: Identification and characterization of murine adipose tissue-derived somatic stem cells of Shenque (CV8) acupoint
    Article Snippet: Materials used in this study were listed as follows: Dulbecco's modified eagle medium (DMEM)-low glucose (HyClone, Logan, UT, USA); DMEM/F12 culture medium (HyClone); B27 supplement without vitamin A (Gibco, New York, NY, USA); recombinant murine epidermal growth factor (EGF) (PeproTech, Rocky Hill, NJ, USA); recombinant murine fibroblast growth factor (FGF)-basic (PeproTech); 0.5% Oil Red O solution (Solarbio, Beijing, China); Masson Stain Kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, Jiangsu, China); cell proliferation assay kit (KeyGen Biotech Co., Ltd., Nanjing, Jiangsu, China); mouse osteogenesis induction and differentiation base media and mouse adipogenesis induction and differentiation base media (Cyagen Biosciences Co., Ltd., Guangzhou, Guangdong, China); anti-mouse Ly-6A/E (stem cell antigen-1, Sca-1) phycoerythrin (PE), anti-mouse CD34 fluorescein isothiocyanate (FITC), anti-mouse CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) FITC, anti-human/mouse CD44 PE (eBioscience, San Diego, CA, USA); anti-nestin antibody, goat anti-mouse immunoglobulin G (IgG)-FITC (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP) (Jackson ImmunoResearch, West Grove, PA, USA); and Alizarin Red S solution (Solarbio, Beijing, China).

    Article Title: Systemic Evaluation of Chimeric LNA/2′-O-Methyl Steric Blockers for Myotonic Dystrophy Type 1 Therapy
    Article Snippet: Three days after plating, the muscle fibers were removed from the dish, and the “plating medium” was replaced with “proliferation medium” consisting of 20% fetal bovine serum (FBS; Thermo Fisher Scientific), 10% HS, 1% CEE, and 2.5 ng/mL recombinant murine fibroblast growth factor-basic (FGF-b; PeproTech) in DMEM (1 × P-S/1 × GlutaMAX).

    Article Title: Identification and characterization of murine adipose tissue-derived somatic stem cells of Shenque (CV8) acupoint
    Article Snippet: Materials used in this study were listed as follows: Dulbecco’s modified eagle medium (DMEM)-low glucose (HyClone, Logan, UT, USA); DMEM/F12 culture medium (HyClone); B27 supplement without vitamin A (Gibco, New York, NY, USA); recombinant murine epidermal growth factor (EGF) (PeproTech, Rocky Hill, NJ, USA); recombinant murine fibroblast growth factor (FGF)-basic (PeproTech); 0.5% Oil Red O solution (Solarbio, Beijing, China); Masson Stain Kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, Jiangsu, China); cell proliferation assay kit (KeyGen Biotech Co., Ltd., Nanjing, Jiangsu, China); mouse osteogenesis induction and differentiation base media and mouse adipogenesis induction and differentiation base media (Cyagen Biosciences Co., Ltd., Guangzhou, Guangdong,China); anti-mouseLy-6A/E (stem cell antigen1, Sca-1) phycoerythrin (PE), anti-mouse CD34 fluorescein isothiocyanate (FITC), anti-mouse CD31 (platelet endothelial cell adhesionmolecule-1, PECAM-1) FITC, anti-human/ mouse CD44 PE (eBioscience, San Diego, CA, USA); antinestin antibody, goat anti-mouse immunoglobulin G (IgG)FITC (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP) (Jackson ImmunoResearch, West Grove, PA, USA); and Alizarin Red S solution (Solarbio, Beijing, China).

    Article Title: Intramuscular Evaluation of Chimeric Locked Nucleic Acid/2′ O Methyl-Modified Antisense Oligonucleotides for Targeted Exon 23 Skipping in Mdx Mice
    Article Snippet: Three days after plating, the muscle fibres were removed from the dish and the ‘plating medium’ was replaced with ‘proliferation medium’ consisting of 20% fetal bovine serum (FBS; Thermo Fisher Scientific), 10% HS, 1% CEE and 2.5 ng/mL recombinant murine fibroblast growth factor-basic (FGF-b; PeproTech, London, UK) in DMEM (1× PS/1× glutamax).

    Article Title: Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation
    Article Snippet: Cell suspensions were cultured in enrichment medium-DMEM/F12 medium supplemented with 2% B27 (Gibco, Grand Island, NY), 20 ng/ml recombinant murine epidermal growth factor (EGF, PeproTech, Rocky Hill, NJ), and 20 ng/ml recombinant murine fibroblast growth factor-basic (FGF, PeproTech, Rocky Hill, NJ) at 37°C under 5% CO humidified condition.

    Article Title: Astragaloside IV Exerts Cognitive Benefits and Promotes Hippocampal Neurogenesis in Stroke Mice by Downregulating Interleukin-17 Expression via Wnt Pathway
    Article Snippet: NSCs were cultured in Neurobasal medium supplemented with 2% B27, 1% N2, 2% Gln, 20 ng/ml recombinant murine epidermal growth factor (EGF, Peprotech, Cat# 315-09), and 20 ng/ml recombinant murine fibroblast growth-basic factor (FGF, Peprotech, Cat# 450-33) for 7–10 days ( ).

    Recombinant:

    Article Title: Akebia saponin D protects hippocampal neurogenesis from microglia-mediated inflammation and ameliorates depressive-like behaviors and cognitive impairment in mice through the PI3K-Akt pathway
    Article Snippet: These tissues were chopped into 1-mm cubes and digested in 0.125% trypsin (Gibco) for 5 min, then the digestion was stopped with soybean trypsin inhibitor. .. Cells were resuspended in complete DMEM/F12 medium (Gibco) containing 20 ng/ml recombinant murine fibroblast growth factor (FGF; PeproTech, NJ, USA), 20 ng/ml recombinant murine epidermal growth factor (EGF; PeproTech), 1% N-2 supplement (Gibco), and 2% B-27 supplement (Gibco). .. After culture for 7 days, neurospheres were isolated by centrifugation (600 g for 3 min), enzymatically dissociated into a single-cell suspension using 0.125% pancreatin (Sigma-Aldrich), and plated at a density of 5 × 104 cells/cm2 in proliferation medium.



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    Image Search Results


    Gli1 -Cre ERT -YFP mice were subjected to Angiotensin II (AngII)-induced cardiac hypertrophy and fibrosis, as described in the Methods section. (A) Masson’s trichrome staining of 4-week AngII or saline treated cardiac tissue; ECM/collagen deposition shown in blue. Representative 20x images of N=4 in each group are shown. (B) Cardiac tissue sections were immunofluorescently stained with anti-GFP antibody and subjected to label-free SHG imaging to visualize the collagen deposition (Red). YFP + cells were imaged (Green) and overlayed to examine their association with ECM/collagen deposition. Representative 40x images of saline (N=10) and AngII-treated (N=12) tissues are shown. ( C ) Cardiac tissue sections were immunofluorescently stained with anti-GFP (Green), anti-αSMA (Red) and DAPI. Representative 60x images of N=6 in each group are shown. (D-I) Cardiac tissue from 2-week AngII- or saline-treated mice were harvested and prepared for scRNA-seq, as described in the Methods section. (D) Uniform Manifold Approximation and Projection (UMAP) visualization is shown. Blue circle highlights the major cluster that is annotated as AdvSca1-SM and fibroblast (SM-Fib) clusters. (E) scRNA-seq data UMAP plot colored by treatment (Saline - blue; AngII - orange). Red circle highlights the AngII-induced shift. (F) YFP transcript positive cells were highlighted in green in the UMAP plot. (G&H) Composition of Saline and AngII-treated samples were visualized for all cells (G) and YFP + cells (H) of the scRNA-seq data. (I) Pathway analysis for genes up-regulated (top) and down-regulated (bottom) by AngII treatment in YFP + AdvSca1-SM cells.

    Journal: bioRxiv

    Article Title: KLF4 in smooth muscle cell-derived progenitor cells is essential for angiotensin II-induced cardiac inflammation and fibrosis

    doi: 10.1101/2024.06.04.597485

    Figure Lengend Snippet: Gli1 -Cre ERT -YFP mice were subjected to Angiotensin II (AngII)-induced cardiac hypertrophy and fibrosis, as described in the Methods section. (A) Masson’s trichrome staining of 4-week AngII or saline treated cardiac tissue; ECM/collagen deposition shown in blue. Representative 20x images of N=4 in each group are shown. (B) Cardiac tissue sections were immunofluorescently stained with anti-GFP antibody and subjected to label-free SHG imaging to visualize the collagen deposition (Red). YFP + cells were imaged (Green) and overlayed to examine their association with ECM/collagen deposition. Representative 40x images of saline (N=10) and AngII-treated (N=12) tissues are shown. ( C ) Cardiac tissue sections were immunofluorescently stained with anti-GFP (Green), anti-αSMA (Red) and DAPI. Representative 60x images of N=6 in each group are shown. (D-I) Cardiac tissue from 2-week AngII- or saline-treated mice were harvested and prepared for scRNA-seq, as described in the Methods section. (D) Uniform Manifold Approximation and Projection (UMAP) visualization is shown. Blue circle highlights the major cluster that is annotated as AdvSca1-SM and fibroblast (SM-Fib) clusters. (E) scRNA-seq data UMAP plot colored by treatment (Saline - blue; AngII - orange). Red circle highlights the AngII-induced shift. (F) YFP transcript positive cells were highlighted in green in the UMAP plot. (G&H) Composition of Saline and AngII-treated samples were visualized for all cells (G) and YFP + cells (H) of the scRNA-seq data. (I) Pathway analysis for genes up-regulated (top) and down-regulated (bottom) by AngII treatment in YFP + AdvSca1-SM cells.

    Article Snippet: Sorted cells were plated in gelatin-coated plates with AdvSca1-SM media (α MEM [Gibco, Cat# 32571036]), 10% MSC qualified fetal bovine serum (FBS)(Thermofisher Cat #12662029), 1x Penicillin Streptomycin, 1ng/mL murine basic fibroblast growth factor (R&D systems 3139-FB), and 5ng/mL murine epidermal growth factor (R&D systems 2028-EG) at the density of 20,000/cm 2 .

    Techniques: Staining, Saline, Imaging